Structural, chemical and nutrient characterization of original feed stock and co-products from a newly built biofuel plant in Saskatchewan
Hettiarachchi Gamage, Inoka
The objective of this study was to determine the structural, chemical and nutrient variation associated with different batches of original feedstock grain (wheat) and co-products (wheat distillers dried grain with solubles, wDDGS) from bioethanol processing and to study possibility of using molecular spectroscopy FT/IR-ATR technique as a rapid tool of identifying the structure spectral differences among the batches. The samples were collected from different batches of wheat and wDDGS produced from the same and newly build bioethanol plant in western Canada with updated bioethanol processing technology.. The results of chemical composition analysis showed significant differences (P<0.05) among the batches of wheat and among the batches of wDDGS. The variation in term of range among the batches of wheat for crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) were 2.1, 2.5 and 1.0 %DM, respectively. Among the batches of wDDGS, the variation in CP, NDF and ADF were 3.9, 5.2 and 3.8 %DM, respectively. The soluble crude protein (SCP) content ranged by 7.2% of CP among the batches of wheat and by 4.2% of CP among the batches of wDDGS. In mineral profiles, variation among the batches of wheat and wDDGS in sulfur were 0.03 and 0.34 % of DM, respectively. In mineral profiles, higher phosphorus and sulfur content associated with wDDGS could be a concern. The sulfur content ranged by 0.3 % of DM among the batches of wDDGS with a mean of 1.1 %DM. For the estimated subfractions with Cornell Net Carbohydrate and Protein System (CNCPS), the immediately rumen available PA fraction (NPN) ranged by 14.8 % of CP among the batches of wheat and by 2.7 % of CP among batches of wDDGS. The particle size analysis revealed the small particle size in all three batches of wDDGS with the geometric mean of ranging from 650-690 µm. In situ rumen degradation kinetic results showed a significant differences (P<0.05) among both wheat and wDDGS batches. Multivariate molecular spectral analyses showed a potential of using FT/IR molecular vibrational spectroscopy as a rapid method to identify structure difference associated with different batches. In conclusion, the structural, chemical and nutrient availability vary among the batches of wheat and among the batches of wDDGS. The molecular spectroscopy shows a potential as a rapid tool to identify batch difference in chemical and nutrient profiles and to detect the response of functional group to bioethanol processing. Further study is needed to analyse molecular spectral characteristics in details for development of a successful identification tool to identify batch differences. The variation among the batches of wDDGS should be considered in ration formulation especially at high inclusion rates (40-50% wDDGS in diet DM) even the co-products are produced from the same bioethanol plant.
DegreeMaster of Science (M.Sc.)
DepartmentAnimal and Poultry Science
CommitteeChristensen, David A.; Penner, Greg; Buchanan, Fiona; Pozniak, Curtis
Copyright DateAugust 2012
Wheat, Dried-distillers-grains with solubles, Nutrients, Spectroscopy