A survey of arbuscular mycorrhizal fungi associated with spring wheat cultivars
Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with many plants, including wheat. Typically this association benefits plant growth. This study assessed the AMF communities found in the rhizosphere of field grown spring wheat representing modern (CDC Teal), historic (Red Fife) and ancient (PI 167549) varieties. Spores of AMF were extracted from rhizosphere soil by wet sieving, decanting and sucrose density centrifugation and separated into six distinct size classes ( 53-212 um). Spore morphotypes within each size class were grouped according to visual similarity and photographed at 66X magnification under a stereomicroscope. The number of AMF spores isolated from rhizosphere soil for the wheat cultivars ranged from 328 to 464 per 50g and exhibited a variety of morphotypes. Our preliminary results suggest that historic wheat cultivars support a more numerous and diverse AMF community than modern wheat cultivars.
The following license files are associated with this item: