The purpose of this study is to investigate nitrogen release from various split rate applications of seed-placed environmentally smart nitrogen (ESN) and spring-applied dribble banded Agrotain-UAN (urea and ammonium nitrate solution) and broadcast Agrotain-urea in winter wheat. Cool spring soil conditions have the potential to limit nitrogen (N) release from ESN potentially limiting grain yield potential. Plant root simulator (PRS™) probes were used to monitor N soil supply. It was discovered that N supply did not vary significantly between the split-rate treatments. There were also no significant differences between grain yield and protein content. These results suggest that N release from ESN was not limited by cool spring temperatures and allowed plant vigour similar to traditional spring-applied methods.