This article highlights the efficacy of plant growth regulators and nutrient solutions on root development and survival of ascending purple milkvetch (APMV) cuttings. Three auxin-like growth regulators and Hoagland solutions were used as root inducing agents. The cuttings were planted in sand medium that allowed evaluation of rooting effectiveness. The study showed that up to 100% establishment of APMV cuttings can be achieved with the application of half-strength modified Hoagland (MHL) solution and plant growth regulators such as indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) at 100mg L-1 strength. To the best knowledge of the authors, this is first time documentation of the vegetative propagation of APMV.