Ascochyta resistance in lentil (Lens culinaris Medikus)
The genetic basis of resistance to Ascochyta blight was determined for 20 lentil genotypes and six F2 populations. Plants were space planted in rows in the field with adjacent spreader rows of the susceptible check Spanish Brown. Plants were inoculated by spreading infested crop debris and mist irrigation was used to create epiphytotic conditions. The disease reaction was measured by rating leaf symptoms on a 1-9 scale and by plating random samples of seed from each F2 plant on PDA agar to estimate percent seed-borne infection. Lines PI 339283, PI 374118, ILL 5588 and PR 86-360 had high resistance levels with less than 7.5% seed-borne infection. Laird, Eston, CDC-Richlea and other lines were susceptible with seed-borne infection as high as 57%. Indianhead lentil was moderately resistant. Analysis of F2 data from the six segregating populations suggests that ILL 5588 has one dominant gene and Indianhead has one recessive gene for resistance to Ascochyta blight of lentil.
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