RAPD markers for Ascochyta resistance in lentil
Genetic linkage of RAPDmarkers with genes for resistance to Ascochyta blight was studied in two crosses of lentil. DNA was extracted from F, plants of both crosses (ILL 5588 x Eston and Indianhead x Eston). Screening for Ascochyta was performed on F,-derived F, lines in a disease nursery with a susceptible check. Bulked segregant analysis was used to identify polymorphic RAPD bands in four bulks for 60 primers. Two polymorphic RAPD bands ( one each in primer #333 and 362 in the resistant bulk) were detected in ILL 5588 x Eston population, indicating linkage with the dominant gene, RuZI for Ascochyta resistance. Another polymorphic RAPD band (#338 in the susceptible bulk) was detected in Indianhead x Eston population, indicating linkage with the recessive gene, ra12. These marker linkages must be confirmed by studying individual plants so that they can be useful in the selection program for Ascochyta resistance in lentil.
bulked segregant analysis
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